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1

Low-mutation-rate, reduced-genomeEscherichia coli: an improved host for faithful maintenance of engineered genetic constructs

Bálint Csörgő, Tamás Fehér, Edit Tímár, Frederick R Blattner, György Pósfai

Journal:

Microbial Cell Factories

Year:

2012

Language:

english

File:

PDF, 294 KB

english, 2012

2

Reduced evolvability ofEscherichia coliMDS42, an IS-less cellular chassis for molecular and synthetic biology applications

Kinga Umenhoffer, Tamás Fehér, Gabriella Balikó, Ferhan Ayaydin, János Pósfai, Frederick R Blattner, György Pósfai

Journal:

Microbial Cell Factories

Year:

2010

Language:

english

File:

PDF, 2.80 MB

english, 2010

3

10.1038/35065664

Perna, Nicole T., Plunkett, Guy, Burland, Valerie, Mau, Bob, Glasner, Jeremy D., Rose, Debra J., Mayhew, George F., Evans, Peter S., Gregor, Jason, Kirkpatrick, Heather A., Pósfai, György, Hackett, Je

Journal:

Year:

2001

Language:

english

File:

PDF, 1.21 MB

english, 2001

4

10.1038/35065672

Perna, Nicole T., Plunkett, Guy, Burland, Valerie, Mau, Bob, Glasner, Jeremy D., Rose, Debra J., Mayhew, George F., Evans, Peter S., Gregor, Jason, Kirkpatrick, Heather A., Pósfai, György, Hackett, Je

Journal:

Year:

2001

Language:

english

File:

PDF, 207 KB

english, 2001

5

10.1038/35065666

Perna, Nicole T., Plunkett, Guy, Burland, Valerie, Mau, Bob, Glasner, Jeremy D., Rose, Debra J., Mayhew, George F., Evans, Peter S., Gregor, Jason, Kirkpatrick, Heather A., Pósfai, György, Hackett, Je

Journal:

Year:

2001

Language:

english

File:

PDF, 237 KB

english, 2001

6

Characterization of cycA mutants of Escherichia coli: An assay for measuring in vivo mutation rates

Tamás Fehér, Botond Cseh, Kinga Umenhoffer, Ildikó Karcagi, György Pósfai

Journal:

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis

Year:

2006

Language:

english

File:

PDF, 300 KB

english, 2006

7

Bacteriophage recombineering in the lytic state using the lambda red recombinases

Tamás Fehér, Ildikó Karcagi, Frederick R. Blattner, György Pósfai

Journal:

Microbial Biotechnology

Year:

2012

Language:

english

File:

PDF, 467 KB

english, 2012

8

A broad-host-range in vivo pop-out and amplification system for generating large quantities of 50-to 100-kb genomic fragments for direct DNA sequencing

Jadwiga Wild, Zdenka Hradečná, György Pósfai, Waclaw Szybalski

Journal:

Year:

1996

Language:

english

File:

PDF, 762 KB

english, 1996

9

Cre/loxP-mediated in vivo excision of large segments from yeast genome and their amplification based on the 2 μm plasmid-derived system

Young Geol Yoon, György Pósfai, Waclaw Szybalski, Sun Chang Kim

Journal:

Year:

1998

Language:

english

File:

PDF, 366 KB

english, 1998

10

In the fast lane: Large-scale bacterial genome engineering

Tamás Fehér, Valerie Burland, György Pósfai

Journal:

Journal of Biotechnology

Year:

2012

Language:

english

File:

PDF, 820 KB

english, 2012

11

Structure of the Bacillus sphaericus R modification methylase gene

György Pósfai, Antal Kiss, Sára Erdei, János Pósfai, Pál Venetianer

Journal:

Journal of Molecular Biology

Year:

1983

Language:

english

File:

PDF, 3.82 MB

english, 1983

12

The dawn of evolutionary genome engineering

Pál, Csaba, Papp, Balázs, Pósfai, György

Journal:

Nature Reviews Genetics

Year:

2014

Language:

english

File:

PDF, 1.12 MB

english, 2014

13

A simple method for locating methylated bases in DNA, as applied to detect asymmetric methylation by M FokIA

Pósfai, György, Szybalski, Waclaw

Journal:

Year:

1988

Language:

english

File:

PDF, 474 KB

english, 1988

14

A simple method for locating methylated bases in DNA using class-IIS restriction enzymes

Pósfai, György, Szybalski, Waclaw

Journal:

Year:

1988

Language:

english

File:

PDF, 246 KB

english, 1988

15

A novel gene-fusing vector: construction of a 5′-GGmCC-specific chimeric methyltransferase, M · BspRI/M · BsuRI

Kim, Sun C., Pósfai, Gyorgy, Szybalski, Waclaw

Journal:

Year:

1991

Language:

english

File:

PDF, 1.24 MB

english, 1991

16

Overproduction of the Bacillus sphaericus R modification methylase in Escherichia coli and its purification to homogeneity

Posfai, Gyorgy, Kiss, Antal, Venetianer, Pal

Journal:

Year:

1986

Language:

english

File:

PDF, 409 KB

english, 1986

17

The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434

Nikolnikov, Sergej, Pósfai, György, Sain, Béla

Journal:

Year:

1984

Language:

english

File:

PDF, 397 KB

english, 1984

18

High-copy-number derivatives of the plasmid cloning vector pBR322

Boros, Imre, Pósfai, György, Venetianer, Pál

Journal:

Year:

1984

Language:

english

File:

PDF, 306 KB

english, 1984

19

Systematic Genome Reductions: Theoretical and Experimental Approaches

Fehér, Tamás, Papp, Balázs, Pál, Csaba, Pósfai, György

Journal:

Chemical Reviews

Year:

2007

Language:

english

File:

PDF, 603 KB

english, 2007

20

DNA Vaccines Free from Mobile DNA

Frisch, David, Novy, Robert, Landry, Charles, Kirkpatrick, Heather, Pósfai, György, Blattner, Frederick R.

Journal:

Genetic Engineering & Biotechnology News

Year:

2013

Language:

english

File:

PDF, 331 KB

english, 2013

21

In vivo excision and amplification of large segments of the Escherichia coli genome

Pósfai, György, Koob, Michael, Hradečná, Zdenka, Hasan, Noaman, Filutowicz, Marcin, Szybalski, Waclaw

Journal:

Nucleic Acids Research

Year:

1994

Language:

english

File:

PDF, 1.31 MB

english, 1994

22

Structure of the gene coding for the sequence-specific DNA-methyltransferase of the B. subtilis phage SPR

Pósfai, György, Baldauf, Frank, Erdei, Sára, Pósfai, Jánós, Venetianer, Pál, Kiss, Antal

Journal:

Nucleic Acids Research

Year:

1984

Language:

english

File:

PDF, 2.03 MB

english, 1984

23

Predictive motifs derived from cytosine methyltransferases

Pósfai, János, Bhagwat, Ashok S., Pósfai, György, Roberts, Richard J.

Journal:

Nucleic Acids Research

Year:

1989

Language:

english

File:

PDF, 881 KB

english, 1989

24

Nudeotide sequence of the BsuRI restriction-modification system

Kiss, Antal, Posfai, Gyorgy, Keller, Christopher C., Venetianer, Pal, Roberts, Richard J.

Journal:

Nucleic Acids Research

Year:

1985

Language:

english

File:

PDF, 1.46 MB

english, 1985

25

A highly precise and portable genome engineering method allows comparison of mutational effects across bacterial species

Nyerges, Ákos, Csörgő, Bálint, Nagy, István, Bálint, Balázs, Bihari, Péter, Lázár, Viktória, Apjok, Gábor, Umenhoffer, Kinga, Bogos, Balázs, Pósfai, György, Pál, Csaba

Journal:

Proceedings of the National Academy of Sciences

Year:

2016

Language:

english

File:

PDF, 875 KB

english, 2016

26

Complementation by detached parts of GGCC-specific DNA methyltransferases

Pósfai, György, Kim, Sun C., Szilák, László, Kovács, Attila, Venetianer, Pál

Journal:

Nucleic Acids Research

Year:

1991

Language:

english

File:

PDF, 4.10 MB

english, 1991

27

System-level genome editing in microbes

Csörgő, Bálint, Nyerges, Ákos, Pósfai, György, Fehér, Tamás

Journal:

Current Opinion in Microbiology

Year:

2016

Language:

english

File:

PDF, 1.02 MB

english, 2016

28

Genome-Wide Abolishment of Mobile Genetic Elements Using Genome Shuffling and CRISPR/Cas-Assisted MAGE Allows the Efficient Stabilization of a Bacterial Chassis

Umenhoffer, Kinga, Draskovits, Gábor, Nyerges, Ákos, Karcagi, Ildikó, Bogos, Balázs, Tímár, Edit, Csörgő, Bálint, Herczeg, Róbert, Nagy, István, Fehér, Tamás, Pál, Csaba, Pósfai, György

Journal:

ACS Synthetic Biology

Year:

2017

Language:

english

File:

PDF, 2.48 MB

english, 2017

29

Enhancing the translational capacity of E. coli by resolving the codon bias

Lipinszki, Zoltán, Vernyik, Viktor, Farago, Nora, Sari, Tobias, Puskas, Laszlo G, Blattner, Frederick, Posfai, Gyorgy, Gyorfy, Zsuzsanna

Journal:

ACS Synthetic Biology

Year:

2018

Language:

english

File:

PDF, 3.41 MB

english, 2018